US 12,404,517 B2
Rhizobial tRNA-derived small RNAs and uses thereof for regulating plant nodulation
Jianxin Ma, West Lafayette, IN (US); Bo Ren, Beijing (CN); Jingbo Duan, West Lafayette, IN (US); and Xutong Wang, West Lafayete, IN (US)
Assigned to Purdue Research Foundation, West Lafayette, IN (US)
Appl. No. 17/611,802
Filed by Purdue Research Foundation, West Lafayette, IN (US)
PCT Filed May 18, 2020, PCT No. PCT/US2020/033464
§ 371(c)(1), (2) Date Nov. 16, 2021,
PCT Pub. No. WO2020/232448, PCT Pub. Date Nov. 19, 2020.
Claims priority of provisional application 62/848,672, filed on May 16, 2019.
Prior Publication US 2022/0251589 A1, Aug. 11, 2022
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8262 (2013.01) [C12N 15/8218 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A Glycine max plant, plant root, or plant cell comprising a recombinant polynucleotide construct encoding at least one or more small RNA fragments, wherein each small RNA fragment downregulates a target gene(s) of the Glycine max plant, plant root, or plant cell that are negative regulators of nodulation such that the Glycine max plant produces increased nodules when inoculated with a Bradyrhizobium or Sinorhizobium species or strain as compared to a corresponding Glycine max plant, root, or cell that does not comprise the recombinant polynucleotide construct, wherein:
(a) the target gene is GmRHD3a which encodes the amino acid sequence of SEQ ID NO: 80, GmRHD3b which encodes the amino acid sequence of SEQ ID NO: 81, GmHAM4a which encodes the amino acid sequence of SEQ ID NO: 82, GmHAM4b which encodes the amino acid sequence of SEQ ID NO: 83, or GmLRX5 which encodes the amino acid sequence of SEQ ID NO: 84,
or
(b) the target genes are GmRHD3a which encodes the amino acid sequence of SEQ ID NO: 80 and GmRHD3b which encodes the amino acid sequence of SEQ ID NO: 81, or GmHAM4a which encodes the amino acid sequence of SEQ ID NO: 83 and GmHAM4b which encodes the amino acid sequence of SEQ ID NO: 83.