US 12,404,504 B2
Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis
Jeremy Minshull, Los Altos, CA (US); Sridhar Govindarajan, Los Altos, CA (US); and Maggie Lee, San Jose, CA (US)
Assigned to DNA TWOPOINTO INC., Newark, CA (US)
Filed by DNA TWOPOINTO INC., Newark, CA (US)
Filed on Jun. 4, 2021, as Appl. No. 17/339,607.
Application 17/339,607 is a continuation of application No. 16/842,707, filed on Apr. 7, 2020, granted, now 11,060,086.
Claims priority of provisional application 62/978,862, filed on Feb. 20, 2020.
Claims priority of provisional application 62/873,346, filed on Jul. 12, 2019.
Claims priority of provisional application 62/831,103, filed on Apr. 8, 2019.
Prior Publication US 2021/0292719 A1, Sep. 23, 2021
Int. Cl. C12N 15/11 (2006.01); C12N 5/071 (2010.01); C12N 9/00 (2006.01); C12N 9/12 (2006.01)
CPC C12N 15/11 (2013.01) [C12N 5/0602 (2013.01); C12N 9/1241 (2013.01); C12Y 207/07 (2013.01)] 17 Claims
 
1. A polynucleotide comprising an open reading frame encoding a transposase, the amino acid sequence of which is at least 90% identical to SEQ ID NO: 39, operably linked to a heterologous promoter, wherein the amino acid sequence comprises at least 2 mutations selected from S41V, L43S, V81E, V81P, D83S, V85L, P125S, Q126S, Q131R, Q131T, S136V, E140C, E140A, 1149C, N151Q, K152L, L153F, D155T, T162I, K169E, N212S, V238F, I239L, H241N, S264R, T268D, T268E, V280P, T297C, L299F, M300R, M305N, L312I, C316A, N322T, H357R, K360Q, K396R, K397S, Y421F, H430Y, A447N, A447D, A449S, A449V, V450I, K476L, R485K, V492A, Y495F, L507I, H512N, L585K, S589T, T595K and Q603K, relative to the sequence of SEQ ID NO: 39.