US 12,404,496 B2
Bioreactor systems
Ethan S. Lippmann, Nashville, TN (US); Alejandra I. Romero-Morales, Nashville, TN (US); Brian J. O'Grady, Nashville, TN (US); Kylie M. Balotin, Nashville, TN (US); Leon M. Bellan, Nashville, TN (US); and Vivian Gama, Nashville, TN (US)
Assigned to Vanderbilt University, Nashville, TN (US)
Appl. No. 17/623,391
Filed by Vanderbilt University, Nashville, TN (US)
PCT Filed Jun. 28, 2020, PCT No. PCT/US2020/040026
§ 371(c)(1), (2) Date Dec. 28, 2021,
PCT Pub. No. WO2020/264455, PCT Pub. Date Dec. 30, 2020.
Claims priority of provisional application 62/868,332, filed on Jun. 28, 2019.
Prior Publication US 2022/0364061 A1, Nov. 17, 2022
Int. Cl. C12N 5/071 (2010.01); C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12M 1/32 (2006.01); C12M 1/34 (2006.01)
CPC C12N 5/0697 (2013.01) [C12M 23/12 (2013.01); C12M 27/06 (2013.01); C12M 41/14 (2013.01); C12M 41/42 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A bioreactor system, comprising:
a first frame and a second frame connected to the first frame,
the first frame defining a well plate inset and standoff insets for a first set of metal standoffs;
a well plate positioned within the well plate inset, the well plate defining a plurality of wells;
the second frame defining a plurality of mounts;
the second frame defining a plurality of insets for a second set of metal standoffs,
wherein a polymer collar is coupled to each of the plurality of mounts;
wherein a gear is positioned in each of the plurality of mounts;
wherein each of the plurality of gears is in communication with at least one adjacent gear;
wherein a paddle is coupled to each of the plurality of gears;
wherein each paddle is positioned within one of the plurality of wells;
a motor plate supported by and connected to the first set of metal standoffs and the second set of metal standoffs,
wherein the first set of metal standoffs extend between the first frame and the motor plate,
wherein the second set of metal standoffs extend between the second frame and the motor plate, and
wherein a length of the first set of metal standoffs is greater than a length of the second set of metal standoffs;
a motor mounted on the motor plate, the motor being operatively connected to one of the plurality of gears; and
a controller in communication with the motor, the controller programmed to control a speed of the motor.