	US 12,404,495 B2
	Epithelial cell differentiation of human mesenchymal stromal cells
Laura E. Niklason, Greenwich, CT (US); and Julio J. Mendez, Bridgeport, CT (US)
Assigned to Yale University, New Haven, CT (US)
Filed by YALE UNIVERSITY, New Haven, CT (US)
Filed on May 26, 2021, as Appl. No. 17/331,257.
Application 17/331,257 is a division of application No. 14/911,571, granted, now 11,028,367, previously published as PCT/US2014/050815, filed on Aug. 13, 2014.
Claims priority of provisional application 61/866,570, filed on Aug. 16, 2013.
Prior Publication US 2021/0363492 A1, Nov. 25, 2021
Int. Cl. C12N 5/071 (2010.01); A61K 35/28 (2015.01); A61K 35/36 (2015.01); A61K 35/42 (2015.01)

CPC C12N 5/0688 (2013.01) [A61K 35/36 (2013.01); A61K 35/42 (2013.01); A61K 35/28 (2013.01); C12N 2500/25 (2013.01); C12N 2500/84 (2013.01); C12N 2500/90 (2013.01); C12N 2501/11 (2013.01); C12N 2501/385 (2013.01); C12N 2501/39 (2013.01); C12N 2501/395 (2013.01); C12N 2501/81 (2013.01); C12N 2506/1353 (2013.01); C12N 2506/1384 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01); C12N 2533/90 (2013.01)]

7 Claims

1. A population of epithelial lung cells seeded on a substrate, wherein the cells are differentiated from adipose tissue-derived mesenchymal stem cells (AT-MSCs), wherein the epithelial lung cells comprise at least 95% Clara cells that express at least one epithelial marker comprising CCSP and do not express cytokeratin-5.