US 12,404,413 B2
Bio-ink structures and methods of producing the same
Fang Qian, Santa Cruz, CA (US); Sarah Baker, Dublin, CA (US); Joshua R. Deotte, Livermore, CA (US); Eric Duoss, Dublin, CA (US); Jennifer Marie Knipe, Oakland, CA (US); Samantha Ruelas, Newberry Springs, CA (US); Christopher Spadaccini, Oakland, CA (US); Joshuah K. Stolaroff, Oakland, CA (US); and Cheng Zhu, Livermore, CA (US)
Assigned to LAWRENCE LIVERMORE NATIONAL SECURITY, LLC, Livermore, CA (US)
Filed by Lawrence Livermore National Security, LLC, Livermore, CA (US)
Filed on Oct. 5, 2018, as Appl. No. 16/153,501.
Prior Publication US 2020/0109299 A1, Apr. 9, 2020
Int. Cl. C09D 11/03 (2014.01); B29C 64/106 (2017.01); B29K 105/16 (2006.01); B33Y 10/00 (2015.01); B33Y 70/00 (2020.01); C09D 11/101 (2014.01); C12P 7/06 (2006.01)
CPC C09D 11/03 (2013.01) [B29C 64/106 (2017.08); B33Y 10/00 (2014.12); B33Y 70/00 (2014.12); C09D 11/101 (2013.01); C12P 7/06 (2013.01); B29K 2105/16 (2013.01)] 20 Claims
OG exemplary drawing
 
13. A bio-ink comprising:
a first filler component comprising freeze-dried cells, wherein the first filler component of freeze-dried cells has a cell density of at least about 10 vol % or more of the total volume of the bio-ink,
a second filler component selected from the group consisting of nanocellulose, cellulose nanocrystals (CNC), cellulose nanofibrils (CNF), and nanocellulose crystalline powder,
wherein the bio-ink is configured to be used for bioprinting three-dimensional structures including living cells, and
a binder comprising an oligomer, monomer, or a combination thereof, wherein the binder is configured to form a polymer when the bio-ink is used for bio-printing the three-dimensional structures including living cells.