	US 12,404,340 B2
	Antibodies against signal-regulatory protein alpha and methods of use
Jaume Pons, San Francisco, CA (US); Bang Janet Sim, South San Francisco, CA (US); Hong Wan, Foster City, CA (US); Tracy Chia-Chien Kuo, San Carlos, CA (US); Steven Elliot Kauder, San Mateo, CA (US); William Don Harriman, Alameda, CA (US); and Shelley Izquierdo, Berkeley, CA (US)
Assigned to ALX Oncology Inc., South San Francisco, CA (US)
Filed by ALX Oncology Inc., South San Francisco, CA (US)
Filed on Jun. 2, 2021, as Appl. No. 17/337,180.
Application 17/337,180 is a division of application No. 15/710,798, filed on Sep. 20, 2017, granted, now 11,401,338.
Claims priority of provisional application 62/515,480, filed on Jun. 5, 2017.
Claims priority of provisional application 62/397,752, filed on Sep. 21, 2016.
Prior Publication US 2023/0018821 A1, Jan. 19, 2023
Int. Cl. C07K 16/28 (2006.01); A61K 39/00 (2006.01); G01N 33/68 (2006.01)

CPC C07K 16/2896 (2013.01) [C07K 16/2803 (2013.01); C07K 16/2818 (2013.01); G01N 33/6854 (2013.01); A61K 2039/505 (2013.01); A61K 2039/507 (2013.01); C07K 16/2827 (2013.01); C07K 2317/23 (2013.01); C07K 2317/24 (2013.01); C07K 2317/30 (2013.01); C07K 2317/31 (2013.01); C07K 2317/34 (2013.01); C07K 2317/52 (2013.01); C07K 2317/56 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01); G01N 2333/70503 (2013.01); G01N 2333/70596 (2013.01); G01N 2500/02 (2013.01)]

37 Claims

1. An isolated antibody that binds an extracellular domain of a human SIRP-α polypeptide, wherein the antibody comprises:

(a) a heavy chain variable (VH) domain comprising:

(i) an HVR-H1 sequence comprising the amino acid sequence of SYDMN (SEQ ID NO: 270), SYDVN (SEQ ID NO:221), or SYDLN (SEQ ID NO:313);

(ii) an HVR-H2 sequence comprising the amino acid sequence of LISGSGEIIYYADSVKG (SEQ ID NO: 156); and

(iii) an HVR-H3 sequence comprising the amino acid sequence of ENNRYRFFDD (SEQ ID NO: 157); and

(b) a light chain variable (VL) domain comprising:

(i) an HVR-L1 sequence comprising the amino acid sequence of RASQSVYTYLA (SEQ ID NO:158);

(ii) an HVR-L2 sequence comprising the amino acid sequence of GASSRAT (SEQ ID NO:159); and

(iii) an HVR-L3 sequence comprising the amino acid sequence of QQYYDRPPLT (SEQ ID NO:160).