	US 12,404,326 B2
	Bivalent bispecific antibody and preparation method thereof, coding gene, host cell and composition
Xiao Feng, Jilin (CN); Lei Jin, Jilin (CN); Tao Wang, Jilin (CN); Hongrui Guo, Jilin (CN); Shuang Liu, Jilin (CN); Yuheng Chen, Jilin (CN); Ning Han, Jilin (CN); and Yangqiu Liang, Jilin (CN)
Assigned to CHANGCHUN GENESCIENCE PHARMACEUTICAL CO., LTD., Jilin (CN)
Appl. No. 17/418,856
Filed by CHANGCHUN GENESCIENCE PHARMACEUTICAL CO., LTD., Jilin (CN)
PCT Filed Dec. 26, 2019, PCT No. PCT/CN2019/128582 § 371(c)(1), (2) Date Jun. 28, 2021, PCT Pub. No. WO2020/135555, PCT Pub. Date Jul. 2, 2020.
Claims priority of application No. 201811622069.2 (CN), filed on Dec. 28, 2018.
Prior Publication US 2022/0073610 A1, Mar. 10, 2022
Int. Cl. C07K 16/28 (2006.01)

CPC C07K 16/2803 (2013.01) [C07K 16/2827 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/35 (2013.01); C07K 2317/622 (2013.01); C07K 2317/92 (2013.01)]

8 Claims

1. A bivalent bispecific antibody, comprising

a1) scFv2 that is a single-chain variable fragment (scFv) derived from an antibody that specifically binds to a first antigen, a flexible peptide, a heavy chain constant region CH1 and a partial hinge region of IgG1 in an order of CH1-partial hinge-flexible peptide scFv2, and

b1) scFv1 that is a single-chain variable fragment (scFv) derived from an antibody that specifically binds to a second antigen and a light chain constant region CL in an order of scFv1-CL; or

a2) scFv2 that is a single-chain variable fragment specifically binding to a first antigen, a flexible peptide, a heavy chain constant region CH1 and a partial hinge region of IgG1 in an order of scFv2-flexible peptide-CH1-partial hinge, and

b2) scFv1 that is a single-chain variable fragment specifically binding to a second antigen and a light chain constant region CL in an order of CL-scFv1;

wherein the order is from N-terminal to C-terminal.