US 12,404,321 B2
Conformation-specific epitopes in alpha-synuclein, antibodies thereto and methods related thereof
Neil R. Cashman, Vancouver (CA); Steven S. Plotkin, Vancouver (CA); Xubiao Peng, Beijing (CN); and Johanne Kaplan, Sherborn, MA (US)
Assigned to University of British Columbia, Vancouver (CA); and ProMIS Neurosciences, Inc., Toronto (CA)
Appl. No. 17/283,292
Filed by THE UNIVERSITY OF BRITISH COLUMBIA, Vancouver (CA); and PROMIS NEUROSCIENCES, INC., Toronto (CA)
PCT Filed Oct. 7, 2019, PCT No. PCT/CA2019/051434
§ 371(c)(1), (2) Date Apr. 7, 2021,
PCT Pub. No. WO2020/073121, PCT Pub. Date Apr. 16, 2020.
Claims priority of provisional application 62/864,060, filed on Jun. 20, 2019.
Claims priority of provisional application 62/820,701, filed on Mar. 19, 2019.
Claims priority of provisional application 62/780,599, filed on Dec. 17, 2018.
Claims priority of provisional application 62/742,408, filed on Oct. 7, 2018.
Prior Publication US 2023/0051538 A1, Feb. 16, 2023
Int. Cl. C07K 16/18 (2006.01); A61P 25/00 (2006.01); C12N 15/63 (2006.01); G01N 33/68 (2006.01); A61K 39/00 (2006.01)
CPC C07K 16/18 (2013.01) [A61P 25/00 (2018.01); C12N 15/63 (2013.01); G01N 33/6896 (2013.01); A61K 39/00 (2013.01); C07K 2317/565 (2013.01); C07K 2317/92 (2013.01)] 18 Claims
 
1. An antibody that binds an epitope in EKTKEQ (SEQ ID NO: 1), in misfolded oligomeric alpha-Syn polypeptide,
wherein the antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprising complementarity determining regions CDR-H1, CDR-H2 and CDR-H3, and the light chain variable region comprising complementarity determining regions CDR-L1, CDR-L2 and CDR-L3,
wherein the complementarity determining regions CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2 and CDR-L3 have amino acid sequences of SEQ ID NOs: 61-66, respectively, SEQ ID NOs: 67-72, respectively, SEQ ID NOs: 73-78, respectively, SEQ ID NOs: 79-81, 76, 77, and 84, respectively, SEQ ID NOs: 91-94, 71, and 96, respectively, or amino acid sequences of SEQ ID NOs: 180, 181, 182, 183, 77, and 184 respectively.