US 12,404,316 B2
Transgenic T cell and chimeric antigen receptor T cell compositions and related methods
Michel W. J. Sadelain, New York, NY (US); Justin Gabriel Andre Francois Eyquem, New York, NY (US); and Jorge Mansilla-Soto, Forest Hills, NY (US)
Assigned to Memorial Sloan Kettering Cancer Center, New York, NY (US)
Filed by Memorial Sloan Kettering Cancer Center, New York, NY (US)
Filed on May 25, 2022, as Appl. No. 17/824,650.
Application 17/824,650 is a division of application No. 16/091,494, granted, now 11,377,637, previously published as PCT/US2017/027601, filed on Apr. 14, 2017.
Claims priority of provisional application 62/462,243, filed on Feb. 22, 2017.
Claims priority of provisional application 62/461,677, filed on Feb. 21, 2017.
Claims priority of provisional application 62/323,675, filed on Apr. 16, 2016.
Claims priority of provisional application 62/323,623, filed on Apr. 15, 2016.
Prior Publication US 2022/0411753 A1, Dec. 29, 2022
Int. Cl. C07K 14/725 (2006.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); A61K 40/50 (2025.01); C12N 5/0783 (2010.01); C12N 9/22 (2006.01); C12N 15/63 (2006.01); C12N 15/86 (2006.01)
CPC C07K 14/7051 (2013.01) [A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/4211 (2025.01); C12N 5/0636 (2013.01); C12N 9/22 (2013.01); C12N 15/63 (2013.01); C12N 15/86 (2013.01); A61K 40/50 (2025.01); A61K 2239/31 (2023.05); A61K 2239/38 (2023.05); A61K 2239/48 (2023.05); C12N 2510/00 (2013.01); C12N 2830/001 (2013.01)] 15 Claims
 
1. A method of treating a human subject with CAR therapy in need thereof, comprising administering to the subject a therapeutically effective amount of a human T cell wherein a promoter-less recombinant nucleic acid sequence encoding a CAR is integrated at a site in the genome of the cell, said site being the first exon of the T cell receptor (TCR) alpha chain gene, such that the CAR is expressed under control of the endogenous TCR alpha chain gene promoter, to produce said CAR at the surface of the cell, and wherein integration of the nucleic acid sequence encoding the CAR at said site prevents expression of a functional TCR alpha chain.