	US 12,404,309 B2
	Host cell for producing a protein of interest
Katrien Claes, Vevey (CH); James Graham, Oxford (GB); Sylwia Jozwiak, Slough (GB); and Christoph Kiziak, Visp (CH)
Assigned to LONZA LTD, Visp (CH)
Appl. No. 16/968,719
Filed by LONZA LTD, Visp (CH)
PCT Filed Jan. 30, 2019, PCT No. PCT/EP2019/052218 § 371(c)(1), (2) Date Aug. 10, 2020, PCT Pub. No. WO2019/154686, PCT Pub. Date Aug. 15, 2019.
Claims priority of application No. 18156329 (EP), filed on Feb. 12, 2018.
Prior Publication US 2020/0399325 A1, Dec. 24, 2020
Int. Cl. C07K 14/39 (2006.01); C12N 15/81 (2006.01)

CPC C07K 14/39 (2013.01) [C12N 15/815 (2013.01)]

15 Claims

1. A method of reducing an amount of total endogenous host cell protein (HCP) in a supernatant of a cell culture of a Komagataella phaffii host cell that is engineered to produce a heterologous protein of interest (POI), comprising genetically modifying the host cell to knockout the gene encoding an endogenous host cell protein comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:1 (HCP1), wherein said genetic modification reduces the amount of total endogenous host cell proteins in the supernatant of the cell culture by at least 10% (mol/mol) as compared to the cell culture of a comparable host cell without said genetic modification.