US 12,404,307 B2
Methods and compositions for use of recombinant bacterial effector proteins as anti-inflammatory agents
Frank J. Rauscher, Wayne, PA (US); and Peter Mondics, Newtown Square, PA (US)
Assigned to The Wistar Institute of Anatomy and Biology, Philadelphia, PA (US); and Innate Biologics LLC, Harrisburg, PA (US)
Filed by The Wistar Institute of Anatomy and Biology, Philadelphia, PA (US)
Filed on Mar. 3, 2022, as Appl. No. 17/686,110.
Application 17/686,110 is a continuation of application No. 16/964,762, abandoned, previously published as PCT/US2019/015224, filed on Jan. 25, 2019.
Claims priority of provisional application 62/622,068, filed on Jan. 25, 2018.
Prior Publication US 2022/0306702 A1, Sep. 29, 2022
Int. Cl. C07K 14/255 (2006.01); A61K 38/00 (2006.01); C07K 14/25 (2006.01)
CPC C07K 14/255 (2013.01) [C07K 14/25 (2013.01); A61K 38/00 (2013.01); C07K 2319/03 (2013.01); C07K 2319/30 (2013.01); C07K 2319/70 (2013.01)] 12 Claims
 
1. A composition comprising a set of paired peptides, wherein the set of paired peptides is linked to a protein transduction domain, and wherein the set of paired peptides comprises a first bacterial effector polypeptide linked to a second bacterial effector polypeptide, wherein the protein transduction domain is a YopM protein transduction domain, an SspH1 protein transduction domain, or an 1paH protein transduction domain wherein the protein transduction domain and the set of paired peptides comprise a fusion protein and the amino acid sequence of the fusion protein is at least 85% identical to the sequence set forth in SEQ ID NO. 10, 13, 16, 19, 22, or 24.