US 12,403,472 B2
Method for forming and respectively exporting droplet wrapping single particle in micro-fluidic chip
Teng Xu, Shandong (CN); Bo Ma, Shandong (CN); and Jian Xu, Shandong (CN)
Assigned to QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY, CHINESE ACADEMY OF SCIENCES, Shandong (CN)
Appl. No. 16/764,803
Filed by QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY, CHINESE ACADEMY OF SCIENCES, Shandong (CN)
PCT Filed Nov. 9, 2018, PCT No. PCT/CN2018/114807
§ 371(c)(1), (2) Date May 15, 2020,
PCT Pub. No. WO2019/096070, PCT Pub. Date May 23, 2019.
Claims priority of application No. 201711130429.2 (CN), filed on Nov. 15, 2017.
Prior Publication US 2020/0376490 A1, Dec. 3, 2020
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/502715 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/08 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0457 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A method for forming and exporting single microparticle-encapsulated droplets, wherein the method utilizes a microfluidic chip, the microfluidic chip comprising: a cover layer and a substrate layer; wherein the substrate layer comprises at least one sample channel, and the at least one sample channel comprises a microfluidic channel and a detection cell; and the cover layer comprises a sample injection hole and an oil storage well hole;
wherein the oil storage well hole and the substrate layer form an oil storage well; an inlet of the at least one sample channel is fluidically connected with the sample injection hole, and an outlet of the at least one sample channel is fluidically connected with the oil storage well, wherein the detection cell and the oil storage well hole are connected through a straight channel, the method comprising: (i) injecting an oil phase into the oil storage well; (ii) injecting an aqueous phase containing a microparticle into the at least one sample channel of the microfluidic chip through the sample injection hole; (iii) adjusting liquid flow in the at least one sample channel so that an interface between the microparticle phase and the oil phase in the oil storage well is steadily stationary nearby the outlet of the at least one sample channel; (iv) capturing a target microparticle with the microparticle capture device, moving the microfluidic chip, so that the target microparticle is dragged to a vicinity of the interface between the aqueous phase and oil phase by optical tweezers; (v) adjusting liquid flow in the at least one sample channel to allow the target microparticle to enter the oil storage well and to form liquid droplets encapsulating a single target microparticle; and (iv) exporting the droplets encapsulating the single target microparticle; wherein a method for adjusting liquid flow in the at least one sample channel is selected from the group consisting of gravity-driven adjusting, injection pump-driven adjusting, and peristaltic pump-driven adjusting and combinations thereof, and a method for exporting the droplets encapsulating the single target microparticle is selected from the group consisting of capillary tube exporting, pipette exporting and combinations thereof.