US 12,403,219 B2
Method for producing decellularized material for transplantation and graft composition consisting of biocompatible material including said material
Tetsuya Higami, Hyogo (JP); Kenichiro Hiwatari, Tokyo (JP); Yu Yamaguchi, Tokyo (JP); Haruki Obara, Tokyo (JP); Takuya Kimura, Tokyo (JP); Mitsumasa Homma, Tokyo (JP); Kyohei Ochiai, Tokyo (JP); Keita Kinoshita, Tokyo (JP); and Naoki Morimoto, Tokyo (JP)
Assigned to ADEKA CORPORATION, Tokyo (JP)
Appl. No. 16/615,177
Filed by ADEKA CORPORATION, Tokyo (JP)
PCT Filed May 25, 2018, PCT No. PCT/JP2018/020141
§ 371(c)(1), (2) Date Nov. 20, 2019,
PCT Pub. No. WO2018/221402, PCT Pub. Date Dec. 6, 2018.
Claims priority of application No. 2017-106400 (JP), filed on May 30, 2017.
Prior Publication US 2020/0222589 A1, Jul. 16, 2020
Int. Cl. A61L 27/36 (2006.01); A61L 27/50 (2006.01)
CPC A61L 27/3691 (2013.01) [A61L 27/3625 (2013.01); A61L 27/507 (2013.01); A61L 2430/40 (2013.01)] 1 Claim
OG exemplary drawing
 
1. A method of producing a decellularized transplantation material, comprising steps of:
(a) harvesting a branch-bearing blood vessel from a donor vertebrate animal;
(b) decellularizing the branch-bearing blood vessel; and
(c) adhering, by a protein denaturation treatment, a portion of the branch-bearing blood vessel where a branch has been excised to close the branch-bearing blood vessel,
wherein the steps are performed in the following sequence:
step (c), followed by step (a), followed by step (b), and after the steps (a), (b) and (c), a step of measuring a pressure resistance of the branch-bearing blood vessel to confirm that the closed portion of the branch-bearing blood vessel where the branch had been excised has a pressure resistance of 150 mmHg or more,
wherein the protein denaturation treatment is performed on the portion of the branch-bearing blood vessel where the branch has been excised at a position 3 mm to 5 mm from a bifurcation in the branch-bearing blood vessel,
wherein the protein denaturation treatment is an ultrasound vibration treatment, and
wherein the decellularizing the branch-bearing blood vessel of step (b) contains a high hydrostatic pressure treatment in which a hydrostatic pressure of 50 to 1,500 MPa is applied.