	US 12,403,177 B2
	Silk-based product formulations and methods of use
Scott Delisle, Mansfield, MA (US); and Michael Santos, Mansfield, MA (US)
Assigned to Cocoon Biotech Inc., Mansfield, MA (US)
Filed by Cocoon Biotech Inc., Mansfield, MA (US)
Filed on Mar. 1, 2023, as Appl. No. 18/176,903.
Application 18/176,903 is a continuation of application No. 16/972,021, granted, now 11,633,455, previously published as PCT/US2019/035306, filed on Jun. 4, 2019.
Claims priority of provisional application 62/757,995, filed on Nov. 9, 2018.
Claims priority of provisional application 62/757,984, filed on Nov. 9, 2018.
Claims priority of provisional application 62/717,025, filed on Aug. 10, 2018.
Claims priority of provisional application 62/680,376, filed on Jun. 4, 2018.
Prior Publication US 2023/0263859 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/17 (2006.01); A61K 9/00 (2006.01); A61K 9/08 (2006.01); A61K 45/06 (2006.01); A61K 47/10 (2017.01); A61K 47/26 (2006.01); A61P 27/04 (2006.01)

CPC A61K 38/1767 (2013.01) [A61K 9/0048 (2013.01); A61K 9/08 (2013.01); A61K 45/06 (2013.01); A61K 47/10 (2013.01); A61K 47/26 (2013.01); A61P 27/04 (2018.01)]

6 Claims

1. A method of preparing processed silk fibroin and controlling hydrolysis of silk fibroin, comprising

providing raw silk,

degumming the raw silk in 0.5 M sodium carbonate at a temperature of 80 to 90° C., and for a time of 120 to 480 minutes, to provide degummed silk fibers,

dissolving the degummed silk fibers in 5 to 13 M lithium bromide at a concentration of 10% (w/v) to 30% (w/v), and

removing the lithium bromide by Tangential Flow Filtration to provide the processed silk fibroin,

wherein the combination of sodium carbonate concentration, time and temperature of the degumming control the hydrolysis of the silk fibroin, and

wherein the processed silk fibroin has an average molecular weight of 15 kDa to 55 kDa by HPLC SEC using a Waters BEH200 Protein Standard Mix.