	US 12,403,170 B2
	*Pharmaceutical composition containing Saururus chinensis* fraction, and preparation method thereof**
Kwang-Soon Shin, Gyeonggi-do (KR); and Yang-Kook Rho, Gyeonggi-do (KR)
Assigned to PHARMACOBIO, INC, Gyeonggi-Do (KR); and KYONGGI UNIVERSITY INDUSTRY & ACADEMIA COOPERATION FOUNDATION, Gyeonggi-Do (KR)
Appl. No. 18/010,071
Filed by PHARMACOBIO, INC., Gyeonggi-do (KR); and KYONGGI UNIVERSITY INDUSTRY & ACADEMIA COOPERATION FOUNDATION, Gyeonggi-do (KR)
PCT Filed Jun. 17, 2021, PCT No. PCT/KR2021/007582 § 371(c)(1), (2) Date Dec. 13, 2022, PCT Pub. No. WO2021/256865, PCT Pub. Date Dec. 23, 2021.
Claims priority of application No. 10-2020-0073480 (KR), filed on Jun. 17, 2020.
Prior Publication US 2023/0218701 A1, Jul. 13, 2023
Int. Cl. A61K 36/78 (2006.01); A61P 1/00 (2006.01)

CPC A61K 36/78 (2013.01) [A61P 1/00 (2018.01); A61K 2236/19 (2013.01); A61K 2236/333 (2013.01); A61K 2236/51 (2013.01)]

5 Claims

1. A method for preparing a solvent fraction of Saururus chinensis effective for inflammatory bowel disease, the method comprising the steps of:

(S1) extracting Saururus chinensis with a 50-90 volume % ethanol aqueous solution,

(S2) adding purified water to the residue remaining after the extraction, and heating to 70-100° C.,

(S3) enzymatically treating the result of the step S2,

(S4) removing the residue in the extract and filtering the extract,

(S5) adding ethanol to the filtrate of the step S4 to form a precipitate, and

(S6) obtaining the precipitate.