	US 12,403,169 B2
	*Compositions and methods related to Rhamnus prinoides* (gesho) extract for the inhibition of polymicrobial biofilm formation**
Mariya Campbell, Suwanee, GA (US); and Eric Gilbert, Decatur, GA (US)
Assigned to GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC., Atlanta, GA (US)
Filed by GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC., Atlanta, GA (US)
Filed on Jun. 30, 2023, as Appl. No. 18/345,643.
Application 18/345,643 is a division of application No. 17/053,496, granted, now 11,717,551, previously published as PCT/US2019/031157, filed on May 7, 2019.
Claims priority of provisional application 62/667,910, filed on May 7, 2018.
Prior Publication US 2024/0041967 A1, Feb. 8, 2024
Int. Cl. A61K 36/72 (2006.01); A01N 31/02 (2006.01); A01N 35/02 (2006.01); A01N 37/10 (2006.01); A01N 65/08 (2009.01); A61K 31/045 (2006.01); A61K 31/121 (2006.01); A61K 31/235 (2006.01); A61L 2/00 (2006.01); A61L 2/18 (2006.01); A61L 15/20 (2006.01); A61L 15/40 (2006.01); A61P 31/04 (2006.01); C11D 3/48 (2006.01); A61L 101/34 (2006.01); A61L 101/36 (2006.01); A61L 101/38 (2006.01)

CPC A61K 36/72 (2013.01) [A01N 31/02 (2013.01); A01N 35/02 (2013.01); A01N 37/10 (2013.01); A01N 65/08 (2013.01); A61K 31/045 (2013.01); A61K 31/121 (2013.01); A61K 31/235 (2013.01); A61L 2/0088 (2013.01); A61L 2/18 (2013.01); A61L 15/20 (2013.01); A61L 15/40 (2013.01); A61P 31/04 (2018.01); C11D 3/48 (2013.01); A61L 2101/34 (2020.08); A61L 2101/36 (2020.08); A61L 2101/38 (2020.08); A61L 2202/24 (2013.01); A61L 2300/404 (2013.01); C11D 2111/14 (2024.01)]

23 Claims

1. A method of inhibiting biofilm formation on a surface comprising contacting a surface susceptible to biofilm formation with a composition consisting of 4-hydroxy-4-methylpentanone (HMP) and a pharmaceutically acceptable carrier or a composition consisting of a pharmaceutically acceptable carrier, HMP, and at least one small molecule selected from the group consisting of: an extract of Rhamnus prinoides (gesho), 4-ethoxy benzoic acid (4EB), ethyl 4-ethoxybenzoate (EEB), methyl gallate, methyl paraben, adipic acid, phytol, or phytol acetate; and wherein the biofilm is not caused by Staphylococcus aureus.