US 12,403,167 B2
Phenylethanoid glycoside extract from Acanthus ilicifolius L., preparation method thereof and use as anti-liver injury medicament
Changyun Wang, Shandong (CN); Changlun Shao, Shandong (CN); Mengqi Zhang, Shandong (CN); Hong Bai, Shandong (CN); Xia Ren, Shandong (CN); Shijun Yue, Shandong (CN); Lanting Xin, Shandong (CN); and Qing Zhao, Shandong (CN)
Assigned to OCEAN UNIVERSITY OF CHINA, Shandong (CN)
Filed by OCEAN UNIVERSITY OF CHINA, Shandong (CN)
Filed on Sep. 27, 2021, as Appl. No. 17/485,995.
Application 17/485,995 is a continuation in part of application No. PCT/CN2020/096097, filed on Jun. 15, 2020.
Claims priority of application No. 201911272041.5 (CN), filed on Dec. 12, 2019.
Prior Publication US 2022/0031783 A1, Feb. 3, 2022
Int. Cl. A61K 36/19 (2006.01); A61K 31/192 (2006.01); A61K 31/7032 (2006.01); A61P 1/16 (2006.01)
CPC A61K 36/19 (2013.01) [A61K 31/192 (2013.01); A61K 31/7032 (2013.01); A61P 1/16 (2018.01); A61K 2236/15 (2013.01); A61K 2236/17 (2013.01); A61K 2236/333 (2013.01); A61K 2236/51 (2013.01)] 12 Claims
 
1. A phenylethanoid glycoside extract, wherein the phenylethanoid glycoside extract comprises at least four compounds of acteoside, isocrenatoside, isoacteoside and p-coumaric acid, and the phenylethanoid glycoside extract is prepared by a method comprising following steps:
pulverizing dried whole plant Acanthus ilicifolius L. and passing through a 20-30 mesh sieve to obtain Acanthus ilicifolius L. powder, adding a 50%-95% ethanol solution whose mass is 8-20 times of the powder, soaking for 1-3 h and then extracting under reflux for 2-4 h, filtering and collecting an extract of the powder, and then extracting a filter residue under reflux for 1.5-2 h with 50%-95% ethanol solution whose mass is 8-10 times of the filter residue and collecting an extract of the filter residue, combining the extract of the powder and the extract of the filter residue, concentrating and subjecting to macroporous resin column chromatography, eluting successively by 10%, 30% and 50% ethanol solutions for four column volumes, respectively, collecting a fraction eluted by the 50% ethanol solution and drying to obtain the phenylethanoid glycoside extract, wherein the percentages of ethanol solutions are all volume percentages.