US 12,402,620 B2
Cell cryopreservation pretreatment operation plate
Futoshi Inoue, Fujinomiya (JP); Keiichi Kato, Tokyo (JP); and Satoshi Ueno, Kawasaki (JP)
Assigned to KITAZATO CORPORATION, Fuji (JP)
Filed by Kitazato Corporation, Fuji (JP)
Filed on Feb. 4, 2022, as Appl. No. 17/592,785.
Application 17/592,785 is a continuation of application No. PCT/JP2020/029814, filed on Aug. 4, 2020.
Claims priority of application No. 2019-143967 (JP), filed on Aug. 5, 2019.
Prior Publication US 2022/0151223 A1, May 19, 2022
Int. Cl. A01N 1/00 (2006.01); A01N 1/125 (2025.01); A01N 1/142 (2025.01); C12M 1/00 (2006.01)
CPC A01N 1/142 (2025.01) [A01N 1/125 (2025.01)] 18 Claims
OG exemplary drawing
 
1. A cell cryopreservation pretreatment operation plate comprising:
a planar operation portion;
a first recess for storing a low-concentration treatment solution that contains a cell membrane-permeable cryoprotectant at a low concentration;
a second recess for storing a high-concentration treatment solution that contains a cell membrane-permeable cryoprotectant at a high concentration;
a third recess for storing a high-concentration treatment solution that contains a cell membrane-permeable cryoprotectant at a high concentration; and
a fourth recess for storing a discarded solution,
wherein the planar operation portion includes, on a surface thereof, a first circular segment for placing a cell membrane-permeable cryoprotectant-free treatment solution, a second circular segment for placing the low-concentration treatment solution, and a third circular segment for placing the low-concentration treatment solution,
the planar operation portion further includes, on the surface thereof, an enlarged first circular segment for placing a cell membrane-permeable cryoprotectant-free treatment solution, an enlarged second circular segment for placing the low-concentration treatment solution, and an enlarged third circular segment for placing the low-concentration treatment solution, the enlarged first circular segment enclosing the first circular segment, the enlarged second circular segment enclosing the second circular segment, and the enlarged third circular segment enclosing the third circular segment,
the enlarged first circular segment has a diameter larger than a diameter of the first circular segment and encloses the first circular segment in such a manner that the first circular segment is located inside the enlarged first circular segment and is in contact with an outer edge of the enlarged first circular segment, the enlarged second circular segment has a diameter larger than a diameter of the second circular segment and encloses the second circular segment in such a manner that the second circular segment is located inside the enlarged second circular segment and is in contact with an outer edge of the enlarged second circular segment, the enlarged third circular segment has a diameter larger than a diameter of the third circular segment and encloses the third circular segment in such a manner that the third circular segment is located inside the enlarged third circular segment and is in contact with an outer edge of the enlarged third circular segment,
outer edges of the first circular segment, the second circular segment and the third circular segment are annular grooves, outer edges of the enlarged first circular segment, the enlarged second circular segment and the enlarged third circular segment are annular grooves,
the diameter of the enlarged first circular segment is 1.5 to 3 times the diameter of the first circular segment, and an area of the enlarged first circular segment is 2 to 10 times an area of the first circular segment,
the diameter of the enlarged second circular segment is 1.5 to 3 times the diameter of the second circular segment, and an area of the enlarged second circular segment is 2 to 10 times an area of the second circular segment, and
the diameter of the enlarged third circular segment is 1.5 to 3 times the diameter of the third circular segment, and an area of the enlarged third circular segment 3 is 2 to 10 times an area of the third circular segment.