	US 12,072,333 B2
	Method and apparatus for selective removal of cells from a cell suspension by mechanical lysis
Michael D. Henry, Iowa City, IA (US); Benjamin Krog, Iowa City, IA (US); Sarah Vigmostad, Iowa City, IA (US); and Mike Cable, Iowa City, IA (US)
Assigned to University of Iowa Research Foundation, Iowa City, IA (US); and SynderBio, Inc., Coralville, IA (US)
Appl. No. 16/346,562
Filed by UNIVERSITY OF IOWA RESEARCH FOUNDATION, Iowa City, IA (US); and SYNDERBIO, INC., Coralville, IA (US)
PCT Filed Nov. 2, 2017, PCT No. PCT/US2017/059768 § 371(c)(1), (2) Date May 1, 2019, PCT Pub. No. WO2018/085572, PCT Pub. Date May 11, 2018.
Claims priority of provisional application 62/416,609, filed on Nov. 2, 2016.
Prior Publication US 2019/0265239 A1, Aug. 29, 2019
Int. Cl. G01N 33/569 (2006.01); C12Q 1/6806 (2018.01); G01N 1/28 (2006.01); G01N 1/34 (2006.01); G01N 33/483 (2006.01)

CPC G01N 33/56966 (2013.01) [C12Q 1/6806 (2013.01); G01N 1/286 (2013.01); G01N 33/4833 (2013.01); G01N 2001/2866 (2013.01); G01N 1/34 (2013.01)]

16 Claims

1. A method comprising:

disassociating a population of cells from a tissue sample, the population of cells including a population of one or more viable cells and a population of one or more dead and dying cells;

exposing the population of cells to at least one pulse of fluid shear stress having a force along a conduit wall of from about 500 dyn/cm2 to about 2500 dyn/cm2 to substantially lyse the population of one or more dead or dying cells, leaving the population of one or more viable cells substantially intact; and

collecting the population of one or more viable cells.