US 12,072,274 B2
Impedance flow cytometry methods
Hywel Morgan, Southampton (GB); Daniel Spencer, Southampton (GB); Timothy John Jay Inglis, Crawley (AU); Teagan Paton, Perth (AU); and Kieran Mulroney, Perth (AU)
Assigned to University of Southampton, Southampton (GB); and The University of Western Australia, Crawley (AU)
Appl. No. 17/276,717
Filed by University of Southampton, Southampton (GB); and The University of Western Australia, Crawley (AU)
PCT Filed Sep. 16, 2019, PCT No. PCT/GB2019/052593
§ 371(c)(1), (2) Date Mar. 16, 2021,
PCT Pub. No. WO2020/058682, PCT Pub. Date Mar. 26, 2020.
Claims priority of application No. 1815122 (GB), filed on Sep. 17, 2018.
Prior Publication US 2022/0034781 A1, Feb. 3, 2022
Int. Cl. G01N 15/12 (2024.01); C12Q 1/18 (2006.01); G01N 15/02 (2024.01); G01N 15/0227 (2024.01); G01N 15/10 (2024.01); G01N 15/1031 (2024.01)
CPC G01N 15/12 (2013.01) [C12Q 1/18 (2013.01); G01N 15/0227 (2013.01); G01N 15/1023 (2024.01); G01N 15/1031 (2013.01); G01N 2015/0294 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1029 (2024.01); G01N 2015/103 (2024.01)] 15 Claims
OG exemplary drawing
 
1. A method of antimicrobial agent susceptibility testing comprising:
preparing samples of microorganisms suspended in an electrolyte, comprising a first sample of the microorganisms unexposed to antimicrobial agents and a second sample of the microorganisms exposed to an antimicrobial agent;
adding non-biological particles to each sample;
passing the first sample through an impedance flow cytometer to obtain a first impedance signal representing one or more components of impedance values of the unexposed microorganisms;
passing the second sample through the impedance flow cytometer to obtain a second impedance signal representing one or more components of impedance values of the microorganisms exposed to the antimicrobial agent;
comparing the first impedance signal and the second impedance signal; and
determining a susceptibility of the microorganisms to the antimicrobial agent based on any differences between the first impedance signal and second impedance signal.