US 12,071,711 B2
Method of preparing libraries of template polynucleotides
Niall Anthony Gormley, Cambridge (GB); Geoffrey Paul Smith, Cambridge (GB); David Bentley, Cambridge (GB); Roberto Rigatti, Cambridge (GB); and Shujun Luo, Castro Valley, CA (US)
Assigned to Illumina Cambridge Limited, Cambridge (GB)
Filed by Illumina Cambridge Limited, Cambridge (GB)
Filed on Sep. 9, 2021, as Appl. No. 17/470,434.
Application 17/470,434 is a continuation of application No. 16/373,433, filed on Apr. 2, 2019, granted, now 11,142,789.
Application 16/373,433 is a continuation of application No. 15/173,447, filed on Jun. 3, 2016, granted, now 10,253,359, issued on Apr. 9, 2019.
Application 15/173,447 is a continuation of application No. 14/022,470, filed on Sep. 10, 2013, granted, now 9,376,678, issued on Jun. 28, 2016.
Application 14/022,470 is a continuation of application No. 12/799,172, filed on Apr. 20, 2010, granted, now 8,563,478, issued on Oct. 22, 2013.
Application 12/799,172 is a continuation of application No. 11/486,953, filed on Jul. 14, 2006, granted, now 7,741,463, issued on Jun. 22, 2010.
Claims priority of application No. 0522310 (GB), filed on Nov. 1, 2005.
Prior Publication US 2022/0064721 A1, Mar. 3, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C40B 50/06 (2006.01); C12N 15/10 (2006.01); C12Q 1/6855 (2018.01); C12Q 1/686 (2018.01); C40B 40/06 (2006.01)
CPC C40B 50/06 (2013.01) [C12N 15/1068 (2013.01); C12N 15/1093 (2013.01); C12N 15/1096 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/686 (2013.01); C40B 40/06 (2013.01); Y10T 436/143333 (2015.01)] 20 Claims
OG exemplary drawing
 
1. A method for sequencing a library of nucleic acids, comprising:
(a) obtaining a library of nucleic acids comprising a plurality of double-stranded polynucleotides having identical forked polynucleotide adapters at each end, wherein the forked polynucleotide adapters comprise partially complementary first and second polynucleotide strands that are annealed to each other, and wherein at least one of the first or second polynucleotide strands comprises a nucleotide sequence selected from SEQ ID NO:01, SEQ ID NO:02, or a nucleotide sequence complementary to SEQ ID NO:04; and
(b) sequencing the library of nucleic acids.