US 12,071,664 B2
Optics collection and detection system and method
Adrian Fehr, San Francisco, CA (US); Nathaniel Joseph McCaffrey, Mill Valley, CA (US); and Stephen Turner, Seattle, WA (US)
Assigned to PACIFIC BIOSCIENCES OF CALIFORNIA, INC., Menlo Park, CA (US)
Filed by Pacific Biosciences of California, Inc., Menlo Park, CA (US)
Filed on Sep. 7, 2022, as Appl. No. 17/939,760.
Application 17/939,760 is a continuation of application No. 15/195,493, filed on Jun. 28, 2016, abandoned.
Application 15/195,493 is a continuation of application No. 13/031,146, filed on Feb. 18, 2011, granted, now 9,410,891, issued on Aug. 9, 2016.
Claims priority of provisional application 61/410,189, filed on Nov. 4, 2010.
Claims priority of provisional application 61/387,916, filed on Sep. 29, 2010.
Claims priority of provisional application 61/306,235, filed on Feb. 19, 2010.
Prior Publication US 2023/0175060 A1, Jun. 8, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6874 (2018.01); B01L 3/00 (2006.01); B82Y 20/00 (2011.01); C12Q 1/6825 (2018.01); C12Q 1/6869 (2018.01); G01N 21/03 (2006.01); G01N 21/05 (2006.01); G01N 21/64 (2006.01); G01N 21/75 (2006.01); G01N 21/77 (2006.01); G01N 33/543 (2006.01); G02B 6/122 (2006.01)
CPC C12Q 1/6874 (2013.01) [B01L 3/502707 (2013.01); B01L 3/502715 (2013.01); B82Y 20/00 (2013.01); C12Q 1/6825 (2013.01); C12Q 1/6869 (2013.01); G01N 21/03 (2013.01); G01N 21/0303 (2013.01); G01N 21/05 (2013.01); G01N 21/64 (2013.01); G01N 21/6428 (2013.01); G01N 21/645 (2013.01); G01N 21/6452 (2013.01); G01N 21/6454 (2013.01); G01N 21/6456 (2013.01); G01N 21/648 (2013.01); G01N 21/75 (2013.01); G01N 21/77 (2013.01); G01N 33/54373 (2013.01); G02B 6/1226 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/168 (2013.01); G01N 2021/0346 (2013.01); G01N 2021/6434 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/6441 (2013.01); G01N 2021/6463 (2013.01); G01N 2021/757 (2013.01); G01N 2021/7786 (2013.01); G01N 2201/067 (2013.01); G01N 2201/068 (2013.01); G01N 2201/08 (2013.01); Y10T 436/143333 (2015.01)] 20 Claims
OG exemplary drawing
 
1. An integrated device for analysis of fluorescent species, comprising:
an array of reaction cells comprising nanoscale apertures;
at least one illumination waveguide disposed beneath the array of reaction cells extending across a plurality of reaction cells in the array of reaction cells and illuminating the plurality of reaction cells from below so that fluorescent species in the plurality of reaction cells emit fluorescent light;
an array of optical filter elements disposed below the at least one illumination waveguide and selectively passing the fluorescent light emitted from the plurality of reaction cells, wherein corresponding to each respective reaction cell in the plurality of reaction cells, there are a first filter element and a second filter element, wherein the first filter element is configured to allow fluorescent light from a first signal to pass and reject fluorescent light from a second signal, and the second filter element is configured to allow fluorescent light from the second signal to pass and reject the fluorescent light from the first signal, wherein the first signal is at a first wavelength range and the second signal at a second wavelength range, wherein the second wavelength range is different than the first wavelength range; and
an array of detector elements disposed below the array of optical filter elements to detect the fluorescent light passed through the array of optical filter elements, wherein corresponding to each respective reaction cell in the plurality of reaction cells, there are a first single detector element configured to receive the first signal that passes through the first filter element, and a second single detector element configured to receive the second signal that passes through the second filter element, wherein the first single detector element and the second single detector element each comprise a single pixel;
wherein the device is integrated such that the fluorescent light emitted from the fluorescent species in the array of reaction cells is transmitted to the array of sensor elements without passing through air or free space.