	US 12,071,663 B2
	Semi-permeable arrays for analyzing biological systems and methods of using same
Todd M. Gierahn, Brookline, MA (US); J. Christopher Love, Somerville, MA (US); Travis K. Hughes, Cambridge, MA (US); Marc H. Wadsworth, II, Cambridge, MA (US); Alexander K. Shalek, Lexington, MA (US); and Shaina Carroll, Cambridge, MA (US)
Assigned to Massachusetts Institute of Technology, Cambridge, MA (US)
Appl. No. 16/070,505
Filed by MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US)
PCT Filed Jan. 17, 2017, PCT No. PCT/US2017/013791 § 371(c)(1), (2) Date Jul. 16, 2018, PCT Pub. No. WO2017/124101, PCT Pub. Date Jul. 20, 2017.
Claims priority of provisional application 62/279,500, filed on Jan. 15, 2016.
Prior Publication US 2019/0144936 A1, May 16, 2019
Int. Cl. B01J 19/00 (2006.01); C12Q 1/6874 (2018.01)

CPC C12Q 1/6874 (2013.01) [B01J 19/0046 (2013.01); B01J 2219/00572 (2013.01)]

49 Claims

9. An array container for multiple parallel single-cell biochemical analysis, comprising

an array having a plurality of wells, wherein said array has a functionalized top surface in a planar configuration with the openings of the wells, and

a porous membrane configured to be positioned on the top surface of the array and over the openings of the wells,

wherein the porous membrane and the functionalized top surface of the array can cooperate by establishing molecular bonding so as to substantially isolate each well, or a subset of wells, from adjacent wells, wherein isolation of each well or subset of wells comprises confining cells, macromolecules, or a combination thereof, inside the wells by sealing the loaded wells with the porous membrane, wherein the porous membrane pore size selectively isolates the cells and the macromolecules, while allowing flow of liquid and/or molecules of a certain smaller size through the porous membrane.