US 12,071,653 B2
Methods and systems for nucleic acid sequencing
Linda Lee, Palo Alto, CA (US); Steven Menchen, Fremont, CA (US); Theo Nikiforov, Carlsbad, CA (US); Gilad Almogy, Palo Alto, CA (US); and Florian Oberstrass, Menlo Park, CA (US)
Assigned to Ultima Genomics, Inc., Fremont, CA (US)
Filed by Ultima Genomics, Inc., Newark, CA (US)
Filed on Apr. 4, 2022, as Appl. No. 17/712,765.
Application 17/712,765 is a continuation of application No. 16/822,121, filed on Mar. 18, 2020, granted, now 11,326,203.
Application 16/822,121 is a continuation of application No. PCT/US2018/052980, filed on Sep. 26, 2018.
Claims priority of provisional application 62/564,227, filed on Sep. 27, 2017.
Prior Publication US 2022/0348994 A1, Nov. 3, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6823 (2018.01); C12Q 1/6869 (2018.01); G01N 21/64 (2006.01)
CPC C12Q 1/6823 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01)] 23 Claims
 
1. A method for determining a sequence of a template nucleic acid molecule, comprising:
(a) contacting two or more nucleotide analogs with a growing nucleic acid strand having sequence complementarity with said template nucleic acid molecule, under conditions sufficient to incorporate said two or more nucleotide analogs into said growing nucleic acid strand, wherein said nucleotide analogs comprise a modified phosphate group comprising sulfur or selenium;
(b) reacting at least one of said modified phosphate group of said nucleotide analogs with a labeling reagent comprising a label to generate a label-S-P, label-S-S-P, or label-Se-P group on said growing nucleic acid strand;
(c) detecting one or more signals indicative of said label; and
(d) converting said label-S-P, label-S-S-P, or label-Se-P group to an O═P group on said growing nucleic acid strand.