	US 12,071,645 B2
	Enzymes and applications thereof
Eric Eichhorn, Zurich (CH); Boris Schilling, Knonau (CH); Denis Wahler, Toulouse (FR); Laurent Fourage, Suresnes (FR); and Esther Locher, Duebendorf (CH)
Assigned to GIVAUDAN SA, Vernier (CH)
Filed by Givaudan SA, Vernier (CH)
Filed on Aug. 31, 2022, as Appl. No. 17/900,590.
Application 17/900,590 is a continuation of application No. 17/240,262, filed on Apr. 26, 2021, granted, now 11,466,299.
Application 17/240,262 is a continuation of application No. 16/598,317, filed on Oct. 10, 2019, granted, now 11,021,722, issued on Jun. 1, 2021.
Application 16/598,317 is a continuation of application No. 15/568,945, granted, now 10,472,655, issued on Nov. 12, 2019, previously published as PCT/EP2016/058987, filed on Apr. 22, 2016.
Claims priority of application No. 1507207 (GB), filed on Apr. 24, 2015.
Prior Publication US 2023/0220431 A1, Jul. 13, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 17/04 (2006.01); C07D 307/92 (2006.01); C12N 9/90 (2006.01)

CPC C12P 17/04 (2013.01) [C07D 307/92 (2013.01); C12N 9/90 (2013.01)]

20 Claims

1. A process for preparing (−)-Ambrox or a mixture comprising (−)-Ambrox, wherein a mixture of isomers comprising (3E,7E)-homofarnesol (EEH) is enzymatically converted to (−)-Ambrox or a mixture comprising (−)-Ambrox, wherein the enzymatic conversion is carried out using a squalene hopene cyclase/homofarnesol Ambrox cyclase (SHC/HAC) enzyme having homofarnesol Ambrox cyclase activity and comprising an amino acid sequence that has at least 30% identity to SEQ ID No. 1 under reaction conditions suitable for the production of (−)-Ambrox, and wherein the mixture of isomers comprising EEH is selected from at least one of [(3E,7E) and [(3Z,7E)], [(3E,7E) and (3E,7Z)] or [(3Z,7E), (3E,7E) and (3E,7Z)] also designated as [EE:EZ], [EE:ZE] and [EE:EZ:ZE] respectively, wherein (−)-Ambrox is produced in admixture with at least one or more of the by-products (II) or (IV) and (III)