US 12,071,643 B2
Alpha-amylase variants
Carsten Andersen, Vaerloese (DK); Iben Damager, Vaerloese (DK); Chakshusmathi Ghadiyaram, Bangalore (IN); Rajendra Kulothungan Sainathan, Bangalore (IN); and Padma Venkatachalam Iyer, Mumbai (IN)
Assigned to NOVOZYMES A/S, Bagsvaerd (DK)
Appl. No. 16/482,741
Filed by NOVOZYMES A/S, Bagsvaerd (DK)
PCT Filed Jan. 30, 2018, PCT No. PCT/EP2018/052197
§ 371(c)(1), (2) Date Aug. 1, 2019,
PCT Pub. No. WO2018/141707, PCT Pub. Date Aug. 9, 2018.
Claims priority of application No. 201741003686 (IN), filed on Feb. 1, 2017; and application No. 17163114 (EP), filed on Mar. 27, 2017.
Prior Publication US 2019/0345470 A1, Nov. 14, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/24 (2006.01); C12N 9/28 (2006.01); C12N 15/63 (2006.01)
CPC C12N 9/2417 (2013.01) [C12N 15/63 (2013.01); C12Y 302/01001 (2013.01)] 17 Claims
 
1. A variant of a parent alpha-amylase, wherein said variant comprises:
(i) substitutions corresponding to H1*, G109A, N280S, E391A, and G7A, wherein the amino acid numbering corresponds to the positions of the amino acid sequence set forth in SEQ ID NO: 1,
(ii) at least 90% but less than 100% sequence identity with the amino acid sequence set forth in SEQ ID NO: 2
(iii) has alpha-amylase activity, and
(iv) has improved wash performance compared to the parent amino acid sequence set forth in SEQ ID NO: 2.