	US 12,071,642 B2
	Engineered amylase variants
Chinping Chng, Menlo Park, CA (US); Nikki Dellas, San Carlos, CA (US); Da Duan, Foster City, CA (US); Ravi David Garcia, Los Gatos, CA (US); and Harvinder Chagger Maniar, Hayward, CA (US)
Assigned to Codexis, Inc., Redwood City, CA (US); and Société des Produits Nestlé S.A., Vevey (CH)
Filed by Codexis, Inc., Redwood City, CA (US); and Societe des Produits Nestle S.A., Vevey (CH)
Filed on Sep. 6, 2023, as Appl. No. 18/462,237.
Application 18/462,237 is a division of application No. 17/460,147, filed on Aug. 27, 2021, granted, now 11,767,519.
Claims priority of provisional application 63/071,641, filed on Aug. 28, 2020.
Prior Publication US 2024/0026326 A1, Jan. 25, 2024
Int. Cl. C12N 9/26 (2006.01); A61K 38/47 (2006.01); A61P 1/18 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01)

CPC C12N 9/2414 (2013.01) [A61K 38/47 (2013.01); A61P 1/18 (2018.01); C12Y 302/01001 (2013.01)]

21 Claims

1. A recombinant polynucleotide comprising a polynucleotide sequence encoding a recombinant amylase comprising a polypeptide sequence comprising at least 90% sequence identity to SEQ ID NO: 1728, wherein the polypeptide sequence comprises at least a substitution 272G/K/P/R/S, 394E/G/N/R/S, or 91H, or any combinations thereof, wherein the amino acid positions are relative to SEQ ID NO: 2.