US 12,071,636 B2
Pasteurella multocida strains and vaccines having hyaC and nanP deletions
Robert E Briggs, Boone, IA (US); and Fred M Tatum, Nevada, IA (US)
Assigned to The United States of America, as Represented by the Secretary of Agriculture, Washington, DC (US)
Filed by The United States of America, as represented by the Secretary of Agriculture, Washington, DC (US)
Filed on Sep. 17, 2021, as Appl. No. 17/478,023.
Claims priority of provisional application 63/085,495, filed on Sep. 30, 2020.
Prior Publication US 2022/0098558 A1, Mar. 31, 2022
Int. Cl. C12N 9/16 (2006.01); A61K 39/102 (2006.01); C12N 9/04 (2006.01); A61K 39/00 (2006.01)
CPC C12N 9/0006 (2013.01) [A61K 39/102 (2013.01); C12N 9/16 (2013.01); C12Y 101/01022 (2013.01); C12Y 301/03029 (2013.01); A61K 2039/522 (2013.01); A61K 2039/552 (2013.01)] 10 Claims
 
1. An attenuated Pasteurella multocida (P. multocida) bacterium wherein the P. multocida bacterium is attenuated by comprising a mutated hyaC gene that encodes a non-functional hyaC polypeptide having the amino acid sequence of SEQ ID NO:11 or encodes a polypeptide having an amino acid sequence at least 80% identical to the amino acid sequence of SEQ ID NO: 11 over the length of SEQ ID NO:11 that is in-common with said polypeptide; or comprising a mutated nanP gene that encodes a polypeptide unable to take-up sialic acid from growth media having the amino acid sequence of SEQ ID NO:4, or a polypeptide having an amino acid sequence at least 80% identical to the amino acid sequence of SEQ ID NO:4 over the length of SEQ ID NO:4 that is in-common with said polypeptide.