	US 12,071,629 B2
	Wheat having high levels of beta-glucan
Stephen Alan Jobling, Australian Capital Territory (AU); Damien Paul Belobrajdic, South Australia (AU); and Anthony Richard Bird, South Australia (AU)
Assigned to COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, Acton (AU)
Appl. No. 14/910,577
Filed by Commonwealth Scientific and Industrial Research Organisation, Australia Capital Territory (AU)
PCT Filed Aug. 6, 2014, PCT No. PCT/AU2014/050173 § 371(c)(1), (2) Date Feb. 5, 2016, PCT Pub. No. WO2015/017901, PCT Pub. Date Feb. 12, 2015.
Claims priority of application No. 2013902937 (AU), filed on Aug. 6, 2013; and application No. 2014902241 (AU), filed on Jun. 12, 2014.
Prior Publication US 2016/0251670 A1, Sep. 1, 2016
Int. Cl. C12N 15/82 (2006.01); A01H 6/46 (2018.01); A23L 7/10 (2016.01); C12Q 1/6895 (2018.01)

CPC C12N 15/8245 (2013.01) [A23L 7/10 (2016.08); A23L 7/198 (2016.08); C12Q 1/6895 (2013.01); A23V 2002/00 (2013.01); C12Q 2600/13 (2013.01)]

25 Claims

1. Transgenic wheat grain comprising an exogenous polynucleotide which encodes a CslF6 polypeptide, and comprising between 3% and 8% (w/w) (1,3;1,4)-β-D-glucan (BG) which is characterised by one or both of:

a) a DP3/DP4 ratio between 1.0 and 2.3; and

b) partial water solubility such that between 8.0% and 25% of the BG of the grain is water soluble, as determined by a method that comprises treatment of a sample of wholemeal flour obtained from the grain with (i) 80% ethanol for 1 hour at 80° C., followed by (ii) solubilisation of BG in aqueous buffer for 2 hours at 37° C., and (iii) determination of the level of BG solubilised from the sample.