US 12,071,618 B2
Systems and methods for biomolecule retention
Tural Aksel, Redwood City, CA (US); Stephen Hendricks, Los Gatos, CA (US); Elvis Ikwa, San Leandro, CA (US); Pierre Indermuhle, Berkeley, CA (US); Sadie Ingle, Suttons Bay, MI (US); Christina Inman, San Mateo, CA (US); Parag Mallick, San Mateo, CA (US); Torri Elise Rinker, San Francisco, CA (US); and Steven Tan, San Mateo, CA (US)
Assigned to Nautilus Subsidiary, Inc., Seattle, WA (US)
Filed by Nautilus Subsidiary, Inc., Seattle, WA (US)
Filed on Jan. 18, 2024, as Appl. No. 18/416,639.
Application 18/416,639 is a continuation of application No. 18/361,731, filed on Jul. 28, 2023, granted, now 11,912,990.
Application 18/361,731 is a continuation of application No. 18/050,732, filed on Oct. 28, 2022, granted, now 11,760,997, issued on Sep. 19, 2023.
Application 18/050,732 is a continuation of application No. 17/692,035, filed on Mar. 10, 2022, granted, now 11,505,796, issued on Nov. 22, 2022.
Claims priority of provisional application 63/256,761, filed on Oct. 18, 2021.
Claims priority of provisional application 63/159,500, filed on Mar. 11, 2021.
Prior Publication US 2024/0158786 A1, May 16, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01); B01J 19/00 (2006.01); B82Y 5/00 (2011.01); C12Q 1/6804 (2018.01); C12Q 1/6837 (2018.01)
CPC C12N 15/1093 (2013.01) [B01J 19/0046 (2013.01); B82Y 5/00 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6837 (2013.01); B01J 2219/0061 (2013.01)] 32 Claims
 
1. A method, comprising:
a) providing a solid support comprising a plurality of sites, wherein each individual site of the plurality of sites comprises:
i) one and only one nucleic acid nanostructure coupled to the individual site; and
ii) one and only one polypeptide of interest attached to the one and only one nucleic acid nanostructure;
b) delivering binding reagents to the solid support, thereby coupling binding reagents to polypeptides of interest at sites of the plurality of sites;
c) repeating step b) using second binding reagents instead of the binding reagents, wherein the second binding reagents are different from the binding reagents;
d) for each individual site, detecting presence or absence of coupling of each individual binding reagent and second binding reagent to the polypeptide of interest; and
e) based upon presence or absence of coupling of each individual binding reagent and second binding reagent to the polypeptide of interest, identifying the polypeptide of interest at each individual site of the plurality of sites.