US 12,071,455 B2
Modified bacterial microcompartment shell protein
Andrew R. Hagen, Oakland, CA (US); Cheryl A. Kerfeld, Walnut Creek, CA (US); Nancy B. Sloan, Berkeley, CA (US); Markus Sutter, Oakland, CA (US); and Bryan H. Ferlez, Haslett, MI (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US); and BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY, East Lansing, MI (US)
Filed by The Regents of the University of California, Oakland, CA (US); and BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY, East Lansing, MI (US)
Filed on Aug. 26, 2021, as Appl. No. 17/458,131.
Application 17/458,131 is a continuation of application No. 16/412,133, filed on May 14, 2019, granted, now 11,130,788.
Claims priority of provisional application 62/671,369, filed on May 14, 2018.
Prior Publication US 2021/0395314 A1, Dec. 23, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/195 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); C07K 1/22 (2006.01); C07K 14/315 (2006.01); C12N 15/62 (2006.01)
CPC C07K 14/195 (2013.01) [B01D 15/363 (2013.01); B01D 15/3804 (2013.01); C07K 1/22 (2013.01); C07K 14/315 (2013.01); C12N 15/62 (2013.01); C07K 2319/70 (2013.01)] 11 Claims
 
1. A method for titrating a range of BMC shells with variable permeability, the method comprising:
(a) determining a desired permeability for a BMC shell,
(b) forming a series of BMC shells with a variable amount of a fusion protein, comprising a BMC-P and a first component, relative to the amounts of the BMC-H and BMC-T shell proteins, and
(c) determining the permeability of each BMC shell formed with a specific amount of the fusion protein relative the amounts of the BMC-H and BMC-T shell proteins.