US 12,071,340 B2
Integrated analysis devices and related fabrication methods and analysis techniques
Han Cao, San Diego, CA (US); Michael David Austin, San Diego, CA (US); Parikshit A. Deshpande, San Diego, CA (US); Mark Kunkel, San Diego, CA (US); Alexey Y. Sharonov, San Diego, CA (US); and Michael Kochersperger, San Diego, CA (US)
Assigned to Bionano Genomics, Inc., San Diego, CA (US)
Filed by Bionano Genomics, Inc., San Diego, CA (US)
Filed on Mar. 21, 2022, as Appl. No. 17/700,299.
Application 17/700,299 is a continuation of application No. 16/864,551, filed on May 1, 2020, granted, now 11,292,713.
Application 16/864,551 is a continuation of application No. 15/385,302, filed on Dec. 20, 2016, granted, now 10,654,715, issued on May 19, 2020.
Application 15/385,302 is a continuation of application No. 12/996,410, granted, now 9,533,879, issued on Jan. 3, 2017, previously published as PCT/US2009/046427, filed on Jun. 5, 2009.
Claims priority of provisional application 61/059,399, filed on Jun. 6, 2008.
Prior Publication US 2022/0388838 A1, Dec. 8, 2022
Int. Cl. B01L 99/00 (2010.01); B01L 3/00 (2006.01); B81C 1/00 (2006.01); G01N 21/03 (2006.01); G01N 21/64 (2006.01)
CPC B81C 1/00119 (2013.01) [B01L 3/502761 (2013.01); B01L 2200/0663 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0851 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/168 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0442 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/086 (2013.01); B81B 2201/058 (2013.01); B81C 2201/019 (2013.01); G01N 2021/0346 (2013.01); G01N 2021/6439 (2013.01); Y10T 29/4981 (2015.01)] 20 Claims
OG exemplary drawing
 
1. A method of fabricating an analysis device, comprising:
bonding a first substrate and a second substrate,
the bonding giving rise to an enclosed conduit disposed between the substrates, the enclosed conduit being capable of transporting a fluid therethrough,
wherein the enclosed conduit comprises a first front-end branched channel region and a nanochannel analysis region,
wherein the first front-end branched channel region comprises at least a primary channel characterized as having a cross-sectional dimension of from about 10 nm to about 10,000 nm and at least two secondary channels downstream and divided from the primary channel, wherein the total cross-sectional area of the at least two secondary channels is approximately equal to the cross-sectional area of the primary channel, thus maintaining an approximately constant flow rate wherein the ratio of the cross-sectional dimensions of the primary channel to the at least two secondary channels is in the range of 100 to 10,000, wherein the first front-end branched channel region comprises stacked array of channels having progressively reduced cross sectional dimensions, wherein the channels are connected by one to five levels of forks.