US 12,070,530 B2
Decellularization and functionalization of extracellular matrix biomaterials
Samuel LoPresti, Pittsburgh, PA (US); and Bryan N. Brown, Pittsburgh, PA (US)
Assigned to University of Pittsburgh—Of the Commonwealth System of Higher Education, Pittsburgh, PA (US)
Appl. No. 16/761,371
Filed by University of Pittsburgh—Of the Commonwealth System of Higher Education, Pittsburgh, PA (US)
PCT Filed Nov. 9, 2018, PCT No. PCT/US2018/060051
§ 371(c)(1), (2) Date May 4, 2020,
PCT Pub. No. WO2019/094734, PCT Pub. Date May 16, 2019.
Claims priority of provisional application 62/583,846, filed on Nov. 9, 2017.
Prior Publication US 2020/0360564 A1, Nov. 19, 2020
Int. Cl. A61L 27/36 (2006.01)
CPC A61L 27/3687 (2013.01) [A61L 27/3633 (2013.01); A61L 27/3691 (2013.01); A61L 2430/34 (2013.01)] 13 Claims
 
1. A method of preparing an extracellular matrix (ECM) product, comprising directly contacting tissue or an ECM material with a composition, the composition comprising:
a reactive oxygen species (ROS) selected from hydroxyl radical (OH), Superoxide (O2−), nitric oxide (NO.), thiyl (RS.), forming RSO2. in the presence of oxygen and copper or iron ions, peroxyl (ROO.), lipid peroxyl (LOO.), peroxynitrite (ONOO−), hypochloric acid (HOCl), singlet oxygen (1O2), ozone (O3), and lipid peroxide (LOOH); and/or
a reactive nitrogen species (RNS) selected from nitric oxide, peroxynitrite (ONOO−), peroxynitrous acid (ONOOH), nitroxyl anion (NO−), nitryl chloride (NO2Cl), nitrosyl cation (NO+), nitrogen dioxide (NO2.), dinitrogen trioxide (N2O3), or nitrous acid (HNO2),
thereby producing an ECM product having nitroxidative or oxidative modifications, and comprising at least a two-fold increase in protein carbonyl moieties, protein nitrotyrosine moieties, or protein nitrocysteine moieties as compared to the same tissue or ECM material not contacted with the ROS and/or RNS.