US 12,070,490 B2
Development of dual whole cell-based vaccine against pancreatic cancer
Gavreel Kalantarov, Fort Lee, NJ (US); and Ilya Trakht, Bronx, NY (US)
Assigned to THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, New York, NY (US)
Filed by THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, New York, NY (US)
Filed on Apr. 1, 2021, as Appl. No. 17/220,799.
Application 17/220,799 is a division of application No. 16/082,184, granted, now 11,020,465, previously published as PCT/US2017/020589, filed on Mar. 3, 2017.
Claims priority of provisional application 62/303,965, filed on Mar. 4, 2016.
Prior Publication US 2021/0220458 A1, Jul. 22, 2021
Int. Cl. A61K 39/00 (2006.01); A61K 9/00 (2006.01); A61K 39/395 (2006.01); C07K 16/30 (2006.01); C12N 5/0784 (2010.01); C12N 5/0786 (2010.01)
CPC A61K 39/0011 (2013.01) [A61K 9/0019 (2013.01); A61K 39/395 (2013.01); C07K 16/30 (2013.01); C12N 5/0639 (2013.01); C12N 5/0645 (2013.01); A61K 2039/5154 (2013.01); A61K 2039/5158 (2013.01); A61K 2039/54 (2013.01); A61K 2039/545 (2013.01); A61K 2039/575 (2013.01); C12N 2500/44 (2013.01); C12N 2501/22 (2013.01); C12N 2501/2301 (2013.01); C12N 2501/2304 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/24 (2013.01); C12N 2501/25 (2013.01)] 16 Claims
 
1. A method of stimulating an immune response against a cancer cell-specific antigen in an identified cancer in a subject at risk of having the identified cancer, comprising:
co-administering to the subject an immunologically effective amount of
(a) a whole cancer cell antigen-loaded dendritic cells isolated from a first population of white blood cells cultured under a first set of culture conditions, and
(b) a whole cancer cell antigen-loaded macrophages isolated from a second population of white blood cells cultured under a second set of culture conditions,
wherein the white blood cells are each autologous to the subject, and the whole cancer cell antigen-loaded dendritic cells and the whole cancer cell antigen-loaded macrophages have internalized by phagocytosis a whole cancer cell of the identified cancer in vitro.