	US 12,070,470 B2
	Topoisomerase inhibitor for mimicking the effect of ionizing radiations on T cells
Adeline Torro, Aumelas (FR); and Clémence Franc, Orléans (FR)
Assigned to Novagray, Castelnau-le-Lez (FR)
Appl. No. 15/734,319
Filed by Novagray, Castelnau-le-Lez (FR)
PCT Filed Jun. 7, 2019, PCT No. PCT/EP2019/064969 § 371(c)(1), (2) Date Dec. 2, 2020, PCT Pub. No. WO2019/234227, PCT Pub. Date Dec. 12, 2019.
Claims priority of application No. 18305699 (EP), filed on Jun. 8, 2018.
Prior Publication US 2021/0220381 A1, Jul. 22, 2021
Int. Cl. A61K 31/00 (2006.01); A61K 31/05 (2006.01); A61K 31/352 (2006.01); A61K 31/704 (2006.01); A61K 31/7048 (2006.01); A61K 47/68 (2017.01); C12Q 1/25 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01); G01N 33/573 (2006.01)

CPC A61K 31/7048 (2013.01) [A61K 31/05 (2013.01); A61K 31/352 (2013.01); A61K 31/704 (2013.01); A61K 47/6809 (2017.08); C12Q 1/25 (2013.01); G01N 33/5008 (2013.01); G01N 33/5011 (2013.01); G01N 33/505 (2013.01); G01N 33/56966 (2013.01); G01N 33/573 (2013.01); C12Q 2521/519 (2013.01); G01N 2800/40 (2013.01)]

17 Claims

1. An in vitro method for mimicking the effect of an ionizing radiation on T cells, comprising contacting said T cells with at least one inhibitor of topoisomerase, wherein the T cells are contacted with at least one inhibitor of topoisomerase for at least 50 hours, wherein the method mimics the effect of an ionizing radiation at a dose ranging from 2 Gy to 10 Gy, and wherein the effect is the percentage of T cells apoptosis measured 48 hours after ionizing radiation.