US 11,740,249 B2
Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
Carolyn W. Hitko, Grover Beach, CA (US); Thomas Kirkland, Atascadero, CA (US); Thomas Machleidt, Madison, WI (US); Rachel Friedman Ohana, Madison, WI (US); Matt Robers, Madison, WI (US); and Keith Wood, Mt. Horeb, WI (US)
Assigned to Promega Corporation, Madison, WI (US)
Filed by Promega Corporation, Madison, WI (US)
Filed on Sep. 4, 2018, as Appl. No. 16/121,248.
Application 16/121,248 is a continuation of application No. 14/104,845, filed on Dec. 12, 2013, granted, now 10,067,149.
Claims priority of provisional application 61/794,461, filed on Mar. 15, 2013.
Claims priority of provisional application 61/736,429, filed on Dec. 12, 2012.
Prior Publication US 2019/0064192 A1, Feb. 28, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/66 (2006.01); G01N 33/94 (2006.01); G01N 33/542 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/94 (2013.01) [C12Q 1/66 (2013.01); G01N 33/542 (2013.01); G01N 33/582 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A system comprising:
(a) a bioactive agent conjugated to a fluorophore;
(b) a cell that expresses a protein fusion of: (i) a protein of interest and (ii) a luciferase, wherein the bioactive agent is capable of binding non-covalently to the protein of interest upon interaction therewith, wherein the emission spectrum of the luciferase overlaps with the excitation spectrum of the fluorophore; and
(c) a substrate for the luciferase.