CPC G01N 33/94 (2013.01) [C12Q 1/66 (2013.01); G01N 33/542 (2013.01); G01N 33/582 (2013.01)] | 23 Claims |
1. A system comprising:
(a) a bioactive agent conjugated to a fluorophore;
(b) a cell that expresses a protein fusion of: (i) a protein of interest and (ii) a luciferase, wherein the bioactive agent is capable of binding non-covalently to the protein of interest upon interaction therewith, wherein the emission spectrum of the luciferase overlaps with the excitation spectrum of the fluorophore; and
(c) a substrate for the luciferase.
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