CPC C12Q 1/6806 (2013.01) [C12Q 1/6869 (2013.01)] | 15 Claims |
1. A method for preparing a duplex adapter, comprising:
annealing an elongation strand and a template strand at a complementary region to form a first intermediate duplex adapter product, wherein the template strand comprises:
an identifier sequence,
a first capture label attached to the template strand via a first cut site template, and
a second capture label attached to the template strand via a second cut site;
extending the elongation strand to at least partially duplicate the identifier sequence to form a second intermediate duplex adapter product comprising a first cut site between the identifier sequence and the first capture label;
cutting the second intermediate duplex adapter product at the first cut site to form a third intermediate duplex adapter product and a first cleaved by-product comprising the first capture label;
removing undesired products comprising the first capture label; and
cutting at the second cut site to form the duplex adapter and a second cleaved by-product comprising the second capture label.
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