US 11,739,365 B2
Metal nanoparticles for glucose detection and glucose detection method using the same
Dae Sung Yoon, Seoul (KR); Insu Kim, Seoul (KR); Gyudo Lee, Namyangju-si (KR); Sang Won Lee, Seoul (KR); Dongtak Lee, Seoul (KR); Jae Won Jang, Gwangju (KR); Dongsung Park, Seoul (KR); Heeju Ahn, Seoul (KR); Hyo Gi Jung, Daegu (KR); Hoo Seong Lim, Gwangju (KR); and Yonghwan Kim, Seoul (KR)
Assigned to Korea University Research and Business Foundation, Seoul (KR)
Filed by Korea University Research and Business Foundation, Seoul (KR)
Filed on Jan. 27, 2021, as Appl. No. 17/159,902.
Claims priority of application No. 10-2020-0010914 (KR), filed on Jan. 30, 2020.
Prior Publication US 2021/0238649 A1, Aug. 5, 2021
Int. Cl. C12Q 1/26 (2006.01); G01N 21/78 (2006.01); C12Q 1/28 (2006.01)
CPC C12Q 1/26 (2013.01) [C12Q 1/28 (2013.01); G01N 21/78 (2013.01); G01N 2800/042 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A glucose detection method comprising:
a step of preparing metal nanoparticles for glucose detection comprising: metal nanoparticles with glucose oxidase attached; and a cell membrane isolated from erythrocytes, which surrounds the metal nanoparticles;
a step of mixing the metal nanoparticles with a sample;
a step of inducing a color reaction by adding a colorimetric solution comprising a peroxidase and a chromogenic substrate to the mixture of the metal nanoparticles and the sample; and
a step of detecting color change caused by the color reaction,
wherein the addition ratio of the metal nanoparticles, the sample and the colorimetric solution is 2:1:1.