CPC C12N 15/11 (2013.01) [C07H 19/10 (2013.01); C12Q 1/6816 (2013.01); C07H 21/02 (2013.01); C07H 21/04 (2013.01); C12Y 207/07006 (2013.01); C12Y 207/07007 (2013.01); C12Y 207/07049 (2013.01)] | 24 Claims |
1. A method for identifying regions of open DNA in a cell, the method comprising:
(a) introducing a population of oligonucleotide molecules into the cell, wherein each molecule comprises, from 5′ to 3′, an amplification segment, an index barcode segment, a hybridization segment, and a reversibly terminating nucleotide;
(b) incubating the cell under conditions to allow for the hybridization segments of the population of oligonucleotide molecules to anneal to regions of open DNA;
(c) activating at least a portion of the annealed oligonucleotide molecules to expose an extendable 3′ hydroxy group; and
(d) synthesizing cDNAs from the open DNA by extending the activated oligonucleotide molecules from their extendable 3′ hydroxy groups.
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