CPC C12N 9/1252 (2013.01) [C12Q 1/6844 (2013.01); C12Y 207/07007 (2013.01)] | 7 Claims |
1. A mutant DNA polymerase having increased 5′-3′ strand displacement activity and substantially reduced 5′-3′ exonuclease and endonuclease activity, as compared with a control DNA polymerase, wherein the amino acid sequence of the mutant DNA polymerase has at least 90% sequence identity to SEQ ID NO: 1 or SEQ ID NO: 40, and comprises a mutation in the polymerase domain, at position 515 of SEQ ID NO: 1, wherein the mutation is selected from: T516I and/or T516S, wherein the control DNA polymerase comprises the amino acid sequence of SEQ ID NO: 40.
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