US 11,739,306 B2
Mutant DNA polymerase(s) with improved strand displacement ability
Feruz Kurbanov, Mountain View, CA (US)
Assigned to Roche Molecular Systems, Inc., Pleasanton, CA (US)
Appl. No. 17/274,667
Filed by Roche Molecular Systems, Inc., Pleasanton, CA (US)
PCT Filed Sep. 12, 2019, PCT No. PCT/EP2019/074320
§ 371(c)(1), (2) Date Mar. 9, 2021,
PCT Pub. No. WO2020/053327, PCT Pub. Date Mar. 19, 2020.
Claims priority of provisional application 62/730,908, filed on Sep. 13, 2018.
Prior Publication US 2021/0269779 A1, Sep. 2, 2021
Int. Cl. C12N 9/12 (2006.01); C12Q 1/6844 (2018.01)
CPC C12N 9/1252 (2013.01) [C12Q 1/6844 (2013.01); C12Y 207/07007 (2013.01)] 7 Claims
 
1. A mutant DNA polymerase having increased 5′-3′ strand displacement activity and substantially reduced 5′-3′ exonuclease and endonuclease activity, as compared with a control DNA polymerase, wherein the amino acid sequence of the mutant DNA polymerase has at least 90% sequence identity to SEQ ID NO: 1 or SEQ ID NO: 40, and comprises a mutation in the polymerase domain, at position 515 of SEQ ID NO: 1, wherein the mutation is selected from: T516I and/or T516S, wherein the control DNA polymerase comprises the amino acid sequence of SEQ ID NO: 40.