	US 11,739,303 B2
	Recombinant influenza viruses with stabilized NA
Yoshihiro Kawaoka, Middleton, WI (US); Yuri Furusawa, Tokyo (JP); and Seiya Yamayoshi, Kanagawa (JP)
Assigned to Wisconsin Alumni Research Foundation (WARF), Madison, WI (US); and The University of Tokyo, Tokyo (JP)
Filed by Wisconsin Alumni Research Foundation (WARF), Madison, WI (US); and The University of Tokyo, Tokyo (JP)
Filed on Jan. 22, 2021, as Appl. No. 17/155,625.
Claims priority of provisional application 62/965,225, filed on Jan. 24, 2020.
Prior Publication US 2021/0246432 A1, Aug. 12, 2021
Int. Cl. A61K 39/145 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01)

CPC C12N 7/00 (2013.01) [A61K 39/145 (2013.01); A61K 2039/5252 (2013.01); A61K 2039/552 (2013.01); C12N 2760/16121 (2013.01); C12N 2760/16134 (2013.01); C12N 2760/16151 (2013.01); C12N 2760/16163 (2013.01)]

12 Claims

1. An isolated recombinant influenza virus comprising stabilized NA tetramers and a neuraminidase (NA) viral segment encoding a modified NA monomer that forms virions having the stabilized NA tetramers relative to a corresponding influenza virus having a parental NA viral segment encoding an unmodified NA monomer, wherein the modified NA monomer comprises a modified NA stalk that results in the stabilized NA tetramers, wherein the modified NA has a cysteine at position 48 or position 50 or both positions of 48 and 50 relative to the numbering of NA1 of SEQ ID NO:37.