CPC C07K 14/47 (2013.01) [C12N 9/93 (2013.01)] | 16 Claims |
1. A method for ligating a histone peptide onto a fully assembled recombinant nucleosome, the nucleosome comprising core histones and a polynucleotide wrapped around the core histones; the method comprising:
(a) contacting a histone peptide with a transpeptidase to produce a histone peptide-transpeptidase intermediate, wherein the histone peptide comprises a C-terminal transpeptidase recognition site; and
(b) contacting the histone peptide-transpeptidase intermediate with a fully assembled recombinant nucleosome comprising a modified core histone protein in which the N-terminal tail has been deleted and an amino acid sequence suitable for transpeptidase mediated ligation is added to the N-terminus under conditions sufficient for ligation of the histone peptide to the modified core histone protein;
thereby producing a fully assembled recombinant nucleosome with a histone peptide ligated thereto.
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