CPC C07K 14/005 (2013.01) [A61K 39/12 (2013.01); A61K 39/155 (2013.01); C07K 16/1027 (2013.01); C12N 7/00 (2013.01); A61K 48/00 (2013.01); A61K 2039/505 (2013.01); A61K 2039/53 (2013.01); A61K 2039/6031 (2013.01); C07K 2317/24 (2013.01); C12N 2760/18534 (2013.01)] | 15 Claims |
1. A method of stimulating an immune response to Respiratory syncytial virus (RSV) in a subject comprising administering to the subject a pharmaceutical composition comprising an mRNA molecule having a coding sequence encoding a fusion (F) protein of RSV with a deleted C-terminus (F-del) lacking amino acids 554-574 of native RSV-F, said coding sequence being at least 80% identical to the protein coding portion of the sequence of SEQ ID NO: 33, wherein the mRNA comprises from 5′ to 3′: (i) a 5′-cap structure; (ii) a 5′ untranslated region (UTR); (iii) the coding sequence encoding the F protein; (iv) a 3′ UTR; and (v) a poly(A) sequence, wherein the mRNA molecule is complexed with a cationic or polycationic compound.
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