	US 11,738,054 B2
	Method to improve therapeutic properties of stem cells
Dmitry Klokov, Chalk River (CA); Soji Sebastian, Petawawa (CA); and Yevgeniya Le, Deep River (CA)
Assigned to ATOMIC ENERGY OF CANADA LIMITED/ENERGIE ATOMIQUE DU CANADA LIMITÈE, Chalk River (CA)
Appl. No. 16/631,233
Filed by ATOMIC ENERGY OF CANADA LIMITED/ÈNERGIE ATOMIQUE DU CANADA LIMTÈE, Chalk River (CA)
PCT Filed Jul. 20, 2018, PCT No. PCT/CA2018/050883 § 371(c)(1), (2) Date Jan. 15, 2020, PCT Pub. No. WO2019/014774, PCT Pub. Date Jan. 24, 2019.
Claims priority of provisional application 62/534,905, filed on Jul. 20, 2017.
Prior Publication US 2020/0230180 A1, Jul. 23, 2020
Int. Cl. A61K 35/545 (2015.01); A61K 35/34 (2015.01); A61K 35/44 (2015.01); A61K 41/00 (2020.01); A61P 21/00 (2006.01)

CPC A61K 35/545 (2013.01) [A61K 35/34 (2013.01); A61K 35/44 (2013.01); A61K 41/0057 (2013.01); A61P 21/00 (2018.01)]

13 Claims

1. A method of preconditioning muscle stem cells, the method comprising:

a) providing a sample comprising a plurality of target muscle stem cells;

b) irradiating the target muscle stem cells with a low dose of radiation comprising between 10 and 100 mGy of radiation emitted from a radiation source during an irradiation period to convert the target muscle stem cells to irradiated, preconditioned muscle stem cells suitable for use in a subsequent therapeutic treatment process;

wherein irradiating the target muscle stem cells reduces age-associated decline of at least a first cellular function of each target muscle stem cell.