	US 11,738,046 B2
	Macrophage cell therapy to treat orthopedic injury
Peiman Hematti, Middleton, WI (US); Connie Chamberlain, Monona, WI (US); Anna Elizabeth Breiner Clements, Madison, WI (US); Ray Vanderby, Madison, WI (US); and John A. Kink, Madison, WI (US)
Assigned to WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US)
Filed by Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed on Nov. 2, 2018, as Appl. No. 16/179,298.
Claims priority of provisional application 62/581,128, filed on Nov. 3, 2017.
Prior Publication US 2019/0134090 A1, May 9, 2019
Int. Cl. A61K 35/15 (2015.01); C12N 5/0786 (2010.01); C12N 5/0775 (2010.01); A61K 9/00 (2006.01); A61P 29/00 (2006.01); A61P 21/00 (2006.01)

CPC A61K 35/15 (2013.01) [A61K 9/0019 (2013.01); C12N 5/0645 (2013.01); C12N 5/0663 (2013.01); A61P 21/00 (2018.01); A61P 29/00 (2018.01); C12N 2502/1358 (2013.01)]

10 Claims

1. A method of treating an orthopedic injury to a ligament or a tendon in a subject in need thereof, the method comprising the step of:

administering directly to the injured ligament or tendon of the subject a population of cells comprising bone marrow exosome educated macrophages (BM-EEM),

wherein the BM-EEM comprise isolated peripheral CD14+ macrophages cultured with bone marrow mesenchymal stem cell-derived exosomes, and

wherein administration of the cells to the subject improves mechanical properties of the injured ligament or tendon.