	US 12,066,447 B2
	Method and reagent for quantifying cholesterol in high density lipoprotein 3
Noriyuki Sato, Gosen (JP); and Yasuki Itoh, Gosen (JP)
Assigned to DENKA COMPANY LIMITED, Tokyo (JP)
Filed by DENKA COMPANY LIMITED, Tokyo (JP)
Filed on May 24, 2021, as Appl. No. 17/328,879.
Application 17/328,879 is a division of application No. 14/906,589, granted, now 11,041,869, previously published as PCT/JP2014/069513, filed on Jul. 24, 2014.
Claims priority of application No. 2013-153294 (JP), filed on Jul. 24, 2013.
Prior Publication US 2021/0278424 A1, Sep. 9, 2021
Int. Cl. G01N 33/92 (2006.01); C07C 43/20 (2006.01); C07C 43/215 (2006.01); C12Q 1/60 (2006.01)

CPC G01N 33/92 (2013.01) [C07C 43/20 (2013.01); C07C 43/215 (2013.01); C12Q 1/60 (2013.01); G01N 2405/00 (2013.01)]

8 Claims

1. A reagent for quantifying cholesterol in high-density lipoprotein 3 in a serum, said reagent comprising two or more surfactants which react specifically with high-density lipoprotein 3, and cholesterol oxidase and cholesterol esterase;

wherein said two or more surfactants are selected from the group consisting of polyoxyethylene styrenated phenylethers, and the two or more surfactants are combined so as to provide the total HLB of 13.7 to 14.0 of the combined surfactants;

wherein a total concentration of said surfactants is 0.25 to 1.25 w/v % in terms of the final concentrations; and

wherein said two or more surfactants provide a reaction ratio of serum high-density lipoprotein 3 to high-density lipoprotein 2 (HDL3/HDL2) of 4.6 to 10.6.