US 12,066,435 B2
Microdevice and analysis device
Chikaaki Mizokuchi, Kanagawa (JP); Koji Shigemura, Kanagawa (JP); and Ken Sumiyoshi, Kanagawa (JP)
Assigned to TIANMA JAPAN, LTD., Kanagawa (JP)
Filed by Tianma Japan, Ltd., Kanagawa (JP)
Filed on Jul. 23, 2021, as Appl. No. 17/383,557.
Claims priority of application No. 2020-128025 (JP), filed on Jul. 29, 2020.
Prior Publication US 2022/0034876 A1, Feb. 3, 2022
Int. Cl. G01N 33/543 (2006.01); G01N 21/64 (2006.01); G02B 21/16 (2006.01)
CPC G01N 33/54373 (2013.01) [G01N 21/6428 (2013.01); G01N 21/6456 (2013.01); G02B 21/16 (2013.01); G01N 2021/6439 (2013.01); G01N 2201/062 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A microdevice comprising:
a microchannel to which a measurement target solution containing a measurement target substance is introduced;
an antibody being fixed to at least one sidewall surface of the microchannel and specifically binding to the measurement target substance;
a fluorescence-labeled derivative being specifically bound to the antibody and being acquired by fluorescence-labeling the measurement target substance;
a light blocker blocking excitation light exciting fluorescent light radiated by the fluorescence-labeled derivative; and
a first substrate that the excitation light enters, wherein
the measurement target substance and the fluorescence-labeled derivative specifically bind to the antibody in a competitive manner,
the antibody is fixed to the sidewall surface of the microchannel in a state of specifically binding to the fluorescence-labeled derivative, the light blocker blocks the excitation light entering the fluorescence-labeled derivative specifically binding to the antibody, and
the light blocker is formed of a material absorbing the excitation light and the fluorescent light,
wherein a section of the microchannel in a widthwise direction has a tapered shape narrowing toward the first substrate, and
wherein the light blocker is formed of:
(i) polydimethylsiloxane containing carbon black or ferric oxide, or
(ii) polymethyl methacrylate containing carbon black or ferric oxide.