US 12,066,412 B2
Circulating multidimensional liquid chromatography and application thereof
Tiantian Zhao, Guangzhou (CN); Yajun Chen, Guangzhou (CN); Yehui Zhang, Guangzhou (CN); Yousheng Zhang, Guangzhou (CN); Wenjuan Jiao, Guangzhou (CN); Weifeng Liu, Guangzhou (CN); and Fang Zhou, Guangzhou (CN)
Assigned to SERICULTURE & AGRI-FOOD RESEARCH INSTITUTE GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES, Guangzhou (CN)
Filed by Sericulture & Agri-food Research Institute Guangdong Academy of Agricultural Sciences, Guangzhou (CN)
Filed on Aug. 27, 2023, as Appl. No. 18/456,493.
Claims priority of application No. 202310475119.3 (CN), filed on Apr. 28, 2023.
Prior Publication US 2023/0408456 A1, Dec. 21, 2023
Int. Cl. G01N 30/06 (2006.01); G01N 30/02 (2006.01); G01N 30/60 (2006.01)
CPC G01N 30/06 (2013.01) [G01N 2030/027 (2013.01); G01N 2030/6013 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A circulating multidimensional liquid chromatography, comprising an infusion device, a sampling device, a collecting-resampling device, a chromatographic column switching device and a detecting device which are connected sequentially;
wherein the collecting-resampling device comprises a collecting valve and a sample ring, and the collecting valve is connected with the sample ring;
the sampling device is successively connected with the collecting valve and the chromatographic column switching device;
the detecting device is also connected with the collecting valve;
the collecting valve is a three-slot three-position ten-way valve; a valve of the collecting valve has three working rotation positions; 10 interfaces are evenly distributed annularly on a valve stator; a surface of a rotor is provided with three folded or curved flow slots adjacent to each other; and coverage range of each flow slot comprises three contiguous stator interfaces;
one end of the sample ring is connected with the stator interface not covered by the flow slots in the collecting valve, which is denoted as a first interface, and other interfaces are numbered in a clockwise direction; the other end of the sample ring is connected with a sixth interface; the sixth interface is radially corresponding to the first interface; a third interface is connected with a waste liquid outlet; a fourth interface is connected with the detecting device; an eighth interface is connected with the chromatographic column switching device; a ninth interface is connected with the sampling device; the other interfaces are closed with plugs;
the chromatographic column switching device comprises a column switching valve and a plurality of chromatographic columns connected thereto; and the column switching valve is connected with the eighth interface and the detecting device respectively;
the column switching valve is a double-three-position three-way valve; center and edge of a valve stator of the double-three-position three-way valve are respectively provided with a center interface and an edge interface, respectively; an annular region between the center interface and the edge interface is evenly distributed with inner interfaces and outer interfaces used for connecting both ends of the chromatographic columns respectively; and the surface of a valve rotor 151 of the double-three-position three-way valve is respectively provided with an annular flow slot communicated with the center interface, one of the inner interfaces, one of the outer interfaces, and the edge interface respectively, and the annular flow slot is configured to be capable of keeping covering the edge interface during a rotation process of the valve rotor;
the valve stator of the column switching valve is distributed with 8 interfaces; the interface at the center of the valve stator is denoted as eleventh interface, the interface at the edge of the valve stator is denoted as twelfth interface, and the other interfaces are denoted as thirteenth interface-eighteenth interface respectively;
wherein the eleventh interface in the column switching valve is connected with the eighth interface in the collecting valve; the twelfth interface in the column switching valve is connected with the detecting device; and the other interfaces are connected with a plurality of chromatographic columns respectively.