	US 12,065,706 B2
	Methods for producing corn plants with downy mildew resistance and compositions thereof
Jorgen Costes Abellera, General Santos (PH); Romain Fouquet, Saint-Palais (FR); Vincent Lombard, Ballwin, MO (US); Yule Pan, Chesterfield, MO (US); Jean Jose Somera, General Santos (PH); and Xianghai Ye, O'Fallon, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Aug. 26, 2022, as Appl. No. 17/822,615.
Application 17/822,615 is a division of application No. 17/082,851, filed on Oct. 28, 2020, granted, now 11,459,622.
Application 17/082,851 is a division of application No. 15/261,286, filed on Sep. 9, 2016, granted, now 10,858,709, issued on Dec. 8, 2020.
Claims priority of provisional application 62/216,593, filed on Sep. 10, 2015.
Prior Publication US 2023/0135707 A1, May 4, 2023
Int. Cl. A01H 5/10 (2018.01); A01H 1/04 (2006.01); A01H 6/46 (2018.01); C12Q 1/6895 (2018.01)

CPC C12Q 1/6895 (2013.01) [A01H 1/045 (2021.01); A01H 5/10 (2013.01); A01H 6/4684 (2018.05); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)]

6 Claims

1. A method of creating a population of corn plants or corn seeds resistant to Downy Mildew (DM), said method comprising:

a) genotyping a first population of corn plants or corn seeds for the presence of one or more marker loci linked within about 10 centimorgans (cM) of a DM resistance allele within a DM resistance quantitative trait locus (QTL) DM_8.01;

b) selecting from said first population one or more corn plants or corn seeds comprising said one or more marker loci linked to said DM resistance allele selected from the group consisting of:

SEQ ID NO: 77, comprising an A at position 342;

SEQ ID NO: 78, comprising a C at position 422;

SEQ ID NO: 79, comprising a C at position 54; and

SEQ ID NO: 80, comprising an A at position 832; and

c) producing from said one or more corn plants or corn seeds a second population of corn plants or corn seeds comprising said DM resistance allele at said DM resistance QTL DM_8.01, wherein said second population of corn plants or corn seeds comprises at least one corn plant or corn seed having improved resistance to DM as compared to a corn plant or corn seed lacking said DM resistance allele at said DM resistance QTL DM_8.01.