CPC C12Q 1/6883 (2013.01) [C12Q 1/6827 (2013.01); G16B 20/20 (2019.02); G16B 30/20 (2019.02); G16H 50/30 (2018.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01)] | 21 Claims |
1. A method comprising
isolating single sperm cells from a sperm sample;
constructing single cell RNA-Seq libraries, direct RNA measurement libraries, or cDNA libraries from the isolated single sperm cells;
generating a gene expression profile of each of MIR762HG, RP11-315I20.1, TADA2A, MEST, RP11-480I12.10, TMCO2, ZFP36L1, ATP1B3, CRAMP1, SPTY2D1-AS1, TNP1, AC007557.1, ACAP1, MARCKS, CTD-2568A17.1, PRM1, TCEB2, and TMEM31 based on the constructed single cell RNA-Seq libraries, direct RNA measurement libraries, or cDNA libraries;
comparing expression levels of the genes in the gene expression profile with reference expression levels of the same genes in a control healthy sperm sample;
determining that none of the genes in the expression profile are upregulated compared to the level of expression of the respective same gene in a healthy control sperm sample; and
utilizing the sperm sample for in vitro fertilization.
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